mKate2-P2A-LOCa3
| Vector Name | mKate2-P2A-LOCa3 |
| VectorType | Lentiviral |
| Antibiotic Information | Ampicillin |
| Sequencing Primers | 5′ sequencing primer: CGTGTACTGGCTCCGCCTTTTTCC 3′ sequencing primer: cacatagcgtaaaaggagcaacatag |
| Additional Information | Backbone: Lenti-blast Copy Number: High Strain for propogation: Stbl3 |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg 2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggtcg ccaccgttaa 2881 ccgccaccat ggtgagcgag ctgattaagg agaacatgca catgaagctg tacatggagg 2941 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg 3001 gcacccagac catgagaatc aaggcggtcg agggcggccc tctccccttc gccttcgaca 3061 tcctggctac cagcttcatg tacggcagca aaaccttcat caaccacacc cagggcatcc 3121 ccgacttctt taagcagtcc ttccccgagg gcttcacatg ggagagagtc accacatacg 3181 aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct 3241 acaacgtcaa gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa 3301 cactcggctg ggaggcctcc accgagaccc tgtaccccgc tgacggcggc ctggaaggca 3361 gagccgacat ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttgaagacca 3421 catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggaca 3481 gaagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg 3541 ctgtggccag atactgcgac ctccctagca aactggggca cagacctagg ggcagcggcg 3601 ccaccaactt cagcctgctg aagcaggccg gcgacgtgga ggagaacccc ggccccggta 3661 ccgagctcgg atctggaagc ggtatgcacc cggagcccgc cccgcccccg agccgcagca 3721 gtcccgagct tcccccaagc ggcggcagca ccaccagcgg cagccgccgg agccgccgcc 3781 gcagcgggga cggggagccc ccgggggccc cgccaccgcc gccgtccgcc gtcacctacc 3841 cggactggat cggccagagt tactccgagg tgatgagcct caacgagcac tccatgcagg 3901 cgctgtcctg gcgcaagctc tacttgagcc gcgccaagct taaagcctcc agccggacct 3961 cggctctgct ctccggcttc gccatggtgg caatggtgga ggtgcagctg gacgctgacc 4021 acgactaccc accggggctg ctcatcgcct tcagtgcctg caccacagtg ctggtggctg 4081 tgcacctgtt tgcgctcatg atcagcacct gcatcctgcc caacatcgag gcggtgagca 4141 acgtgcacaa tctcaactcg gtcaaggagt caggatccgg gttggctact acacttgaac 4201 gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc attatattcg 4261 cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg ggaagaaact 4321 gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt agagatgcca 4381 tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt ggtaaaaagt 4441 tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc cagtacttta 4501 ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga gagggagtca 4561 tgctgattaa gaaaactgca gaaaatattg atgaggcggc aaaagaactt gggtccggac 4621 cccatgagcg catgcaccgc gacatcgagc tggcctgggc cttctccacc gtcatcggca 4681 cgctgctctt cctagctgag gtggtgctgc tctgctgggt caagttcttg cccctcaaga 4741 agcagccagg ccagccaagg cccaccagca agccccccgc cagtggcgca gcagccaacg 4801 tcagcaccag cggcatcacc ccgggccagg cagctgccat cgcctcgacc accatcatgg 4861 ttaccttcgg actgatcttt atcgtcttcg ccgtccactt ctaccgctca ctggttagcc 4921 ataagaccga ccgacagttc caggagctca acgagctggc ggagtttgcc cgcttacagg 4981 accagctgga ccacagaggg gaccaccccc tgacgcccgg cagccactat gccctcgagt 5041 aggaattcga tatcaagctt atcggtaatc aacctctgga ttacaaaatt tgtgaaagat 5101 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 5161 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 5221 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 5281 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 5341 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 5401 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 5461 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 5521 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 5581 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 5641 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca 5701 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 5761 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 5821 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 5881 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 5941 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca 6001 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct 6061 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga 6121 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg 6181 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 6241 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 6301 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 6361 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 6421 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 6481 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 6541 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 6601 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 6661 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 6721 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 6781 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 6841 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 6901 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 6961 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 7021 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 7081 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 7141 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 7201 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 7261 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 7321 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 7381 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 7441 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc 7501 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac 7561 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga 7621 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga 7681 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg 7741 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa 7801 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga 7861 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg 7921 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 7981 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 8041 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 8101 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 8161 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 8221 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 8281 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 8341 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 8401 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 8461 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 8521 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8581 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8641 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 8881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 8941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 9001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 9061 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 9121 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 9181 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 9241 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 9301 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 9361 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 9421 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 9481 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 9541 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 9601 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 9661 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 9721 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 9781 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 9841 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 9901 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 9961 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 10021 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 10081 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 10141 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 10201 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 10261 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 10321 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
LOCUS dna 10375 bp FEATURES Location/Qualifiers misc_binding 486..491 /dbxref="REBASE:NdeI" Other Gene 835..1015 /gene="HIV-1_5_LTR other" Other Gene 1126..1170 /gene="HIV-1_psi_pack other" misc_binding 1524..1531 /dbxref="REBASE:NotI" ORF 1558..2445 /sequence="ORF_1 rf(1)" Regulatory_Seq 1680..1913 /gene="RRE reg" misc_binding 2876..2881 /dbxref="REBASE:HpaI" ORF 2889..5042 /sequence="ORF_2 rf(3)" misc_binding 4173..4178 /dbxref="REBASE:BamHI" misc_binding 5043..5048 /dbxref="REBASE:EcoRI" ORF 5157..6206 /sequence="ORF_3 rf(3)" misc_binding 5656..5661 /dbxref="REBASE:ClaI" Other Gene 6180..6360 /gene="HIV-1_5_LTR other" Rep_Origin 6679..6985 /gene="f1 origin" Regulatory_Seq 7095..7330 /gene="SV40ER reg" Promoter 7117..7385 /gene="SV40 prom" Promoter 7237..7438 /gene="SV40 prom" Rep_Origin 7284..7361 /gene="SV40 origin" Promoter 7479..7546 /gene="EM7 prom" Marker 7547..7918 /gene="bleo marker" Other Gene 7547..7921 /gene="sh_ble other" Promoter 8270..8299 /gene="lac prom" Rep_Origin 8608..9227 /gene="pBR322 origin" Marker 9382..10242 /gene="amp marker" ORF 9382..10242 /sequence="ORF_4 rf(5)" Promoter 10284..10312 /gene="amp prom" BASE COUNT 2562 a 2677 c 2743 g 2393 t 0 others ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg 2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggtcg ccaccgttaa 2881 ccgccaccat ggtgagcgag ctgattaagg agaacatgca catgaagctg tacatggagg 2941 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg 3001 gcacccagac catgagaatc aaggcggtcg agggcggccc tctccccttc gccttcgaca 3061 tcctggctac cagcttcatg tacggcagca aaaccttcat caaccacacc cagggcatcc 3121 ccgacttctt taagcagtcc ttccccgagg gcttcacatg ggagagagtc accacatacg 3181 aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct 3241 acaacgtcaa gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa 3301 cactcggctg ggaggcctcc accgagaccc tgtaccccgc tgacggcggc ctggaaggca 3361 gagccgacat ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttgaagacca 3421 catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggaca 3481 gaagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg 3541 ctgtggccag atactgcgac ctccctagca aactggggca cagacctagg ggcagcggcg 3601 ccaccaactt cagcctgctg aagcaggccg gcgacgtgga ggagaacccc ggccccggta 3661 ccgagctcgg atctggaagc ggtatgcacc cggagcccgc cccgcccccg agccgcagca 3721 gtcccgagct tcccccaagc ggcggcagca ccaccagcgg cagccgccgg agccgccgcc 3781 gcagcgggga cggggagccc ccgggggccc cgccaccgcc gccgtccgcc gtcacctacc 3841 cggactggat cggccagagt tactccgagg tgatgagcct caacgagcac tccatgcagg 3901 cgctgtcctg gcgcaagctc tacttgagcc gcgccaagct taaagcctcc agccggacct 3961 cggctctgct ctccggcttc gccatggtgg caatggtgga ggtgcagctg gacgctgacc 4021 acgactaccc accggggctg ctcatcgcct tcagtgcctg caccacagtg ctggtggctg 4081 tgcacctgtt tgcgctcatg atcagcacct gcatcctgcc caacatcgag gcggtgagca 4141 acgtgcacaa tctcaactcg gtcaaggagt caggatccgg gttggctact acacttgaac 4201 gtattgagaa gaactttgtc attactgacc caagattgcc agataatccc attatattcg 4261 cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg ggaagaaact 4321 gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt agagatgcca 4381 tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt ggtaaaaagt 4441 tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc cagtacttta 4501 ttggggttca gttggatgga actgagcatg tccgagatgc tgccgagaga gagggagtca 4561 tgctgattaa gaaaactgca gaaaatattg atgaggcggc aaaagaactt gggtccggac 4621 cccatgagcg catgcaccgc gacatcgagc tggcctgggc cttctccacc gtcatcggca 4681 cgctgctctt cctagctgag gtggtgctgc tctgctgggt caagttcttg cccctcaaga 4741 agcagccagg ccagccaagg cccaccagca agccccccgc cagtggcgca gcagccaacg 4801 tcagcaccag cggcatcacc ccgggccagg cagctgccat cgcctcgacc accatcatgg 4861 ttaccttcgg actgatcttt atcgtcttcg ccgtccactt ctaccgctca ctggttagcc 4921 ataagaccga ccgacagttc caggagctca acgagctggc ggagtttgcc cgcttacagg 4981 accagctgga ccacagaggg gaccaccccc tgacgcccgg cagccactat gccctcgagt 5041 aggaattcga tatcaagctt atcggtaatc aacctctgga ttacaaaatt tgtgaaagat 5101 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 5161 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 5221 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 5281 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 5341 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 5401 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 5461 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 5521 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 5581 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 5641 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca 5701 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 5761 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 5821 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 5881 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 5941 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca 6001 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct 6061 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga 6121 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg 6181 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 6241 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 6301 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 6361 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 6421 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 6481 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 6541 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 6601 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 6661 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 6721 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 6781 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 6841 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 6901 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 6961 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 7021 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 7081 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 7141 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 7201 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 7261 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 7321 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 7381 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 7441 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc 7501 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac 7561 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga 7621 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga 7681 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg 7741 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa 7801 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga 7861 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg 7921 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 7981 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 8041 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 8101 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 8161 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 8221 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 8281 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 8341 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 8401 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 8461 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 8521 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8581 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8641 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 8881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 8941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 9001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 9061 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 9121 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 9181 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 9241 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 9301 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 9361 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 9421 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 9481 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 9541 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 9601 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 9661 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 9721 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 9781 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 9841 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 9901 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 9961 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 10021 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 10081 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 10141 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 10201 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 10261 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 10321 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac //
LOCUS dna 10375 bp FEATURES Location/Qualifiers misc_binding 486..491 /dbxref="REBASE:NdeI" Other Gene 835..1015 /gene="HIV-1_5_LTR other" Other Gene 1126..1170 /gene="HIV-1_psi_pack other" misc_binding 1524..1531 /dbxref="REBASE:NotI" ORF 1558..2445 /sequence="ORF_1 rf(1)" Regulatory_Seq 1680..1913 /gene="RRE reg" misc_binding 2876..2881 /dbxref="REBASE:HpaI" ORF 2889..5042 /sequence="ORF_2 rf(3)" misc_binding 4173..4178 /dbxref="REBASE:BamHI" misc_binding 5043..5048 /dbxref="REBASE:EcoRI" ORF 5157..6206 /sequence="ORF_3 rf(3)" misc_binding 5656..5661 /dbxref="REBASE:ClaI" Other Gene 6180..6360 /gene="HIV-1_5_LTR other" Rep_Origin 6679..6985 /gene="f1 origin" Regulatory_Seq 7095..7330 /gene="SV40ER reg" Promoter 7117..7385 /gene="SV40 prom" Promoter 7237..7438 /gene="SV40 prom" Rep_Origin 7284..7361 /gene="SV40 origin" Promoter 7479..7546 /gene="EM7 prom" Marker 7547..7918 /gene="bleo marker" Other Gene 7547..7921 /gene="sh_ble other" Promoter 8270..8299 /gene="lac prom" Rep_Origin 8608..9227 /gene="pBR322 origin" Marker 9382..10242 /gene="amp marker" ORF 9382..10242 /sequence="ORF_4 rf(5)" Promoter 10284..10312 /gene="amp prom" BASE COUNT 2562 a 2677 c 2743 g 2393 t 0 others